IITA NEWS

90% SAH plantlet survival rate boosting cassava seed availability in Rwanda
May 30, 2024

Rwanda’s cassava sector has faced significant challenges over the years, primarily due to the devastating effects of cassava viral diseases, mainly the Cassava Mosaïc Disease (CMD) and Cassava Brown Streak Disease (CBSD). These two diseases have severely threatened food security and the livelihoods of many cassava farmers nationwide. In 2014/2015, the Kinazi cassava plant, a franchise business established to address market overproduction, nearly shut down due to a shortage of adequate roots for processing. In a desperate bid to alleviate the crisis, the Government of Rwanda (GoR) imported millions of clean stem cuttings from Uganda, providing temporary relief but not a sustainable solution.

Dr Silver Tumwegamire, a Cassava Breeder at IITA, and Sebukeye Andre, a Lab Technician, were assessing the progress of plantlets at the SAH laboratory established at RAB, Rubona Station.
Dr Silver Tumwegamire, a Cassava Breeder at IITA, and Sebukeye Andre, a Lab Technician, were assessing the progress of plantlets at the SAH laboratory established at RAB, Rubona Station.

In response to this crisis, IITA, in collaboration with the Rwanda Agriculture and Animal Resources Development Board (RAB) and other partners, launched efforts in 2017 to counter the negative impact of cassava viral diseases. The efforts include the IFAD-funded CBSD Control Project, the Dutch government-funded Cassava Agribusiness Seed Systems (CASS) Project, and the recent Bill and Melinda Gates Foundation-funded ViRCA Deployment Project. All these initiatives focused on developing and deploying improved resistant varieties and establishing a sustainable clean seed delivery system. These measures have been crucial in halting disease spread and improving farm cassava productivity.

IITA Cassava Breeder and Seed Systems Expert Silver Tumwegamire said, “Recognizing the severe impact of cassava viral diseases on food security, we initiated and implemented several projects to develop and deploy disease-resistant varieties and establish a robust seed system.”

Initially, IITA and RAB established a pre-basic seed multiplication unit, utilizing optimized protocols for micro-propagating virus-indexed tissue culture plants and acclimatization and macro-propagation techniques under screenhouse conditions. However, the hardening of tissue culture (TC) plantlets under cold Rubona station conditions faced low survival rates, complicating efforts to produce adequate numbers swiftly.

Plantlets in the SAH lab at RAB's Rubona Research Station.
Plantlets in the SAH lab at RAB’s Rubona Research Station.

Introducing Semi-autotrophic hydroponics (SAH) technology in 2019 offered a cost-effective and efficient solution. SAH technology significantly improved the survival rates of the hardening plants, making it easier to produce large quantities of clean pre-basic seeds. This technology was introduced through the collaborative support of three projects: the Technologies for African Agricultural Transformation (TAAT), the CBSD Control Project, and the Cassava Agribusiness Seed Systems (CASS) project.

Since its introduction, SAH has revolutionized the production and availability of clean pre-basic seeds in Rwanda. The SAH laboratory, the TC lab, and four large screenhouses collectively comprise a functional pre-basic seed unit under RAB and can produce over 200,000 plantlets or pencil stems per year under a moderate-throughput system.

Athanase Nduwumuremyi, RAB Cassava Breeder and Root Crops Coordinator, explained: “SAH technology has been a game-changer for cassava seed multiplication in Rwanda. It has enabled us to produce high-quality plantlets much faster, ensuring a steady supply of clean seeds for farmers.”

This has tremendously improved multiplication ratios, ensuring an adequate supply of clean plantlets for the next level of early-generation seed multiplication and delivery. From January to April 2024, RAB produced 55,569 pre-basic seeds or plantlets, with a target of reaching 200,000 plantlets by the end of the year. From these plantlets, at least 10 ha of clean essential seeds can be produced annually, yielding at least 2 million stem cuttings for certified one-seed production in the country.

SAH is contributing to an adequate supply chain of cassava clean seeds in Rwanda.
SAH is contributing to an adequate supply chain of cassava clean seeds in Rwanda.

Moreover, RAB supplies plantlets to additional private basic seed multipliers with screenhouses. It is estimated that each establishes at least one hectare of clean basic seed and generates an extra minimum of 0.8 million stem cuttings.

The SAH technology boasts a 90% survival rate for plantlets. In three weeks, 25 plantlets planted in one box can produce 62, which can multiply to 156 after another three weeks, demonstrating a higher multiplication ratio than the two node-cutting multiplication system. SAH’s cost-effective nature, ease of acclimatization, and high survival rates make it a superior alternative to traditional tissue culture and SH micropropagation methods, where only 20% of plantlets may survive.

RAB, supported by IITA, has recently optimized a locally made substrate based on peat moss for micro-propagation under SAH facilities, reducing reliance on the expensive Klansman substrate for macro-propagation. Additionally, RAB is optimizing the one-node cutting macro-propagation technique to enhance the multiplication ratios of pre-basic seeds.

Contributed by Ritha Bumwe


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Olaosebikan, Olamide

Olamide Deborah Nwanze is a Senior Research Associate specializing in gender mainstreaming research to inform participatory and inclusive breeding, variety development, and seed systems policies regarding root, tuber, and banana (RTB) crops in the International Institute of Tropical Agriculture Headquarters, Ibadan, Nigeria. She is a PhD candidate at the University of Ibadan and holds an MSc and B. Agric in Agricultural Extension and Rural Development. Olamide is a fellow of the African Women in Agricultural Research and Development – Gender Responsive Agricultural Systems Policy (AWARD-GRASP) and the Gender Responsive Researchers Equipped for Agricultural Transformation (GREAT). She seeks to apply her gender-mainstreaming learnings, gender-responsive research, and policy development, and review expertise to facilitate women, youth, children, and vulnerable social group’s access to and adoption of agricultural Innovations that guarantee food, nutrition, income, and livelihood security in Africa. Her current roles include mentoring research fellows, development of guides and training manuals, implementation and adaptation of gender frameworks and tools, value chain actors’ engagement as citizen science partners, policy review and redesign, data management, and analysis. Her current research explores mixed methods and participatory and interdisciplinary approaches to capture and highlight gender dynamics, needs, preferences, and challenges stakeholders face within the agricultural livelihood value chain to prioritize gender-inclusive strategies in breeding programs, policy design, and implementation.

At IITA, she contributes to the implementation of the CGIAR Accelerated Breeding Initiative and Genetic Innovation Programs; participatory and gender research within the NextGen CassavaHarvestPlusRTBFoods, and Tricot-ClimMob 1000Farms Projects; and gender knowledge communication via the G-LENS webinar series, video documentary, policy briefspublications, and blogs.

 

Recent publications

de Sousa, K., van Etten, J., Manners, R. Bello, A., Teeken, B., Olaosebikan, O., et al. 2024. The tricot approach: an agile framework for decentralized on-farm testing supported by citizen science. A retrospective. Agron. Sustain. Dev. 44, 8. https://doi.org/10.1007/s13593-023-00937-1

Madu, T., Onwuka, S., Nwafor, S., Onyemauwa, N., Ejechi, M., Ofoeze, M., Ukeje, B., Eluagu, C., Olaosebikan, O., Okoye, B., 2024. Gender-Inclusive Consumer Studies Improve Cassava Breeding in Nigeria in: Frontiers in Sociology, volume 9, number: 1224504, doi: 10.3389/fsoc.2024.1224504

Olaosebikan, O.; Bello, A.; Utoblo, O.; Okoye, B.; Olutegbe, N.; Garner, E.; Teeken, B.; Bryan, E.; Forsythe, L.; Cole, S.; Madu, T. 2023.  Stressors and Resilience within the Cassava Value Chain in Nigeria: Preferred Cassava Variety Traits and Response Strategies of Men and Women to Inform Breeding. Sustainability 15, 7837. https://doi.org/10.3390/su15107837

Okoye, B., Ofoeze, M., Ejechi, M., Onwuka, S., Nwafor, S., Onyemauwa, N., Ukeje, B., Eluagu, C., Obidiegwu, J.*, Olaosebikan, O., Madu, T.* 2023. Prioritizing preferred traits in the yam value chain in Nigeria: a gender situation analysis in: Frontiers in Sociology, volume 8, number: 1232626, pages 1 – 9, ISSN 2297-7775, 2023. https://www.frontiersin.org/articles/10.3389/fsoc.2023.1232626/full

Olaosebikan, O., Bello, A. A., de Sousa, K., Ndjouenkeu, R., Adesokan, M., Alamu, E. O., Agbona, A., van Etten, J., Kegah, F. N.*, Dufour, D., Bouniol, A., Teeken, B. 2023. Consumer acceptability of cassava gari-eba food products across cultural and environmental settings using the triadic comparison of technologies approach (tricot) in: Journal of the Science of Food and Agriculture,

Teeken, B., Olaosebikan, O., Bello, A. A., Madu, T.*, Cole, S. M. 2023. Qualitative assessment on the positionality and preferences of dyads in relation to cassava-related activities: working with tricot trial citizen scientists https://hdl.handle.net/10568/138326 Type: Manual

Bouniol, A., Ceballos, H., Bello, A. A., Teeken, B., Olaosebikan, O., Owoade, D., Agbona, A., Fotso Kuate, A., Madu, T.*, Okoye, B.*, Ofoeze, M.*, Nwafor, S.*, Onyemauwa, N.*, Adinsi, L.*, Forsythe, L., Dufour, D. 2023. Varietal impact on women’s labour, workload and related drudgery in processing root, tuber and banana crops: focus on cassava in sub-Saharan Africa in: Journal of the Science of Food and Agriculture, pages 1 – 25, ISSN 0022-5142,

Bello, A. A., Agbona, A., Olaosebikan, O., Edughaen, G., Dufour, D., Bouniol, A., Iluebbey, P., Ndjouenkeu, R.*, Rabbi, I. Y., Teeken, B. 2023. Genetic and environmental effects on processing productivity and food product yield: drudgery of women’s work in: Journal of the Science of Food and Agriculture, pages 1 – 32, ISSN 0022-5142, 5

Cavicchioli, M., Zimbiti, T., Teeken, B., Nwanze, D.O., Liani, M., Parkes, E. & Bello, A. 2023. IITA-Gender Responsive Breeding Training Report. Genetic Resources Center, IITA-HQ, Ibadan, 21-23 September, 2022. Ibadan, Nigeria: IITA, (34 p.).https://hdl.handle.net/10568/131368

Agbona, A. Peteti P., Teeken B., Olaosebikan O. Bello, A. A., Parkes, E., Rabbi, I. Y., Mueller, L., Egesi, C., Kulakow, P. 2022. Data Management in Multi-disciplinary African RTB Crop Breeding Programs. In: Williamson, H.F., Leonelli, S. (eds) Towards Responsible Plant Data Linkage: Data Challenges for Agricultural Research and Development. Springer, Cham. https://doi.org/10.1007/978-3-031-13276-6_5

Lora Forsythe, Pricilla Marimo, Sarah Mayanja, Olamide D. Olaosebikan, Esme Stuart, Bela Teeken, Benjamin Okoye, Tessy Madu, (2021). Cross-product Gender Analysis of RTBfoods Step 2 Gendered Food Mapping on RTB Products. Understanding the Drivers of Trait Preferences and the Development of Multi-user RTB Product Profiles, WP1. London, UK: RTBfoods Field Scientific Report, 30 p.

Teeken, B., Garner, E., Agbona, A., Balogun, I., Olaosebikan, O., Bello, A., Madu, T., Okoye, B., Egesi, C., Kulakow, P. and Tufan, H.A. 2021. Beyond “Women’s Traits”: Exploring How Gender, Social Difference, and Household Characteristics Influence Trait Preferences. Frontiers Sustainable Food Systems 5:740926. https://doi.org/10.3389/fsufs.2021.740926

Teeken, B., Agbona, A., Bello, A., Olaosebikan, O., Alamu, E., Adesokan, M., Awoyale, W., Madu, T., Okoye, B., Chijioke, U., Owoade, D., Okoro, M., Bouniol, A., Dufour, D., Hershey, C., Rabbi, I., Maziya‐Dixon, B., Egesi, C., Tufan, H. and Kulakow, P. (020. Understanding cassava varietal preferences through pairwise ranking of gari‐eba and fufu prepared by local farmer–processors. Int J Food Sci Technol. https://doi.org/10.1111/ijfs.14862

Bela Teeken, Olamide Olaosebikan, Ireti Balogun, Benjamin Okoye, Tessy Madu, Steven Cole, Abolore Bello, Elizabeth Parkes. (2020). Piloting the G+ customer and product profile tools for gender-responsive cassava breeding in Nigeria. Nigeria: International Institute of Tropical Agriculture (IITA). https://hdl.handle.net/20.500.11766/13000